Identifying Targets of Protective Antibodies against Severe Malaria in Papua, Indonesia, Using Locally Expressed Domains of Plasmodium falciparum Erythrocyte Membrane Protein 1

JS Rambhatla; GQ Tonkin-Hill; E Takashima; T Tsuboi; R Noviyanti; L Trianty; BF Sebayang; DA Lampah; J Marfurt; RN Price; NM Anstey; AT Papenfuss; T Damelang; AW Chung; MF Duffy; SJ Rogerson

Journal article

Identifying Targets of Protective Antibodies against Severe Malaria in Papua, Indonesia, Using Locally Expressed Domains of Plasmodium falciparum Erythrocyte Membrane Protein 1

JS Rambhatla, GQ Tonkin-Hill, E Takashima, T Tsuboi, R Noviyanti, L Trianty, BF Sebayang, DA Lampah, J Marfurt, RN Price, NM Anstey, AT Papenfuss, T Damelang, AW Chung, MF Duffy, SJ Rogerson

Infection and Immunity | AMER SOC MICROBIOLOGY | Published : 2022

DOI: 10.1128/iai.00435-21

Abstract

Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), a diverse family of multidomain proteins expressed on the surface of malaria-infected erythrocytes, is an important target of protective immunity against malaria. Our group recently studied transcription of the var genes encoding PfEMP1 in individuals from Papua, Indonesia, with severe or uncomplicated malaria. We cloned and expressed domains from 32 PfEMP1s, including 22 that were upregulated in severe malaria and 10 that were upregulated in uncomplicated malaria, using a wheat germ cell-free expression system. We used Luminex technology to measure IgG antibodies to these 32 domains and control proteins in 63 individuals (11 chi..

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University of Melbourne Researchers

Gerry Tonkin-Hill Author

Tony Papenfuss Author

Amy Chung Author

Michael Duffy Author

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Grants

Awarded by University of Melbourne

Funding Acknowledgements

Sample collection and analysis were supported by the National Health and Medical Research Council (project grant to M.F.D. [GNT1007954]; program grants to N.M.A., R.N.P. [GNT1037304], and S.J.R. [GNT1092789]; and fellowships to N.M.A. [GNT1135820], A.T.P. [GNT1116955], and A.W.C. [GNT1140509]). Protein expression was partially supported by a JSPS KAKENHI grant, Japan, to E.T. (JP21H02724). R.N.P. is a Wellcome Trust Senior Fellow in Clinical Science (200909). J.S.R. was supported by a Melbourne International Research Scholarship and Melbourne International Fee Remission Scholarship from the University of Melbourne.